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Biofabrications with much promising Bioprinter
The spending on health cares of the the US amounted to 25,095 hundred million US dollars in 2009, accounting for 17.6% of US GDP, 40% of national income and is expected to reach 43,532 hundred million dollars to 2018, acounted for 20.3% GDP. (United States Department of Health and Human Services,
Among these demands, the Biofabrication has consistant increasing contribution in the huge biomedical research and clinical applications. 
Biofabrication is persistently and rapidly developing with the improvement of biomaterials--bioinks and advanced bioprinter technologies, allowing us to build biofabrication systems including for,

1. Bone tissue repair and regeneration
2. Complexed scaffolds for soft tissues
3. Cell proliferation, migration, and tissue formation 
4. Drug testing, discovery and screening
5. Medical aid, wond healing
6. Diseased tissue and oganisim models, etc.


To achieve a realistic time frame for building organized clinically-relevant sizes biofabrication constructs, we Regenovo_ABDC) have extensive emphasis on the Robotic Dispensing Bioprinter, among others types of bioprinters(as that of  the stereolithography (light-curing), electrospinning, laser induced forward transfer, inkjet modules that we could include as addition modules), is the most promising bio-printer both for spatial resolution, print speed, material and cost considerations. 

Table 1. Typical characteristics of three key dispensing approaches in biofabrication

  Laser-induced forward transfer Inkjet printing Robotic dispensing
Resolution ++ + +/-
Fabrication speed - +/- ++
Hydrogel viscosity +/- - +
Gelation speed ++ ++ +/-

Reference: Advanced Materials, 25th Anniversary Article:
Engineering Hydrogels for Biofabrication--3 Hydrogel Based Biofabrication Systems

Base on these key considerations and demands for our bioprinters , we have continous efforts to improve resolution, stability, flexibility and most affordable cost for researchers and clinical developers.  

Features of our Bioprinter

1. Viable cells/biomaterial 3D printing,  most cell survival ratio, much cell than 90%, for longest survival of 4 months, , with global technology leading levels for the living cells printing.   
Based on Robotic dispensing, printable materials are "Unrestricted", as solution, slurry, gel or melt, etc., with high freedom of choices.  

2. The solid state cooling and heating temperature control system
Using an independent solid state cooling elements, and by the liquid heat transfer medium and micro-flow channel network, to realize the  thermal conductivity and temperature control for the print stage and nozzle. The system is highly integrated with precise temperature control, easy extention and operation.  

3. Specially designed unique multi-nozzles technology
Multi-nozzles can be manually or automatically loaded/exchanged for interleaved 3D, multi-material heterogenously located, and multi-layer, multi process steps constructions. 
High-temperature nozzle temperature range 50-260
, low temperature nozzle range for -5-65, can be used to print biological materials that could be melt in between -5 to 260 or in semi-fluid state.
The High-temperature nozzle is manufactured by integrally molded 3D printing technology(SLS), with the optimal design of the heat exchange efficiency, and supports the molten state biological material printing from 50 to 260 .
The Low temperature nozzle can cover -5 (-20 option) to 65 precise temperature control, specially oriented low temperature print way to ensure the vitally active state of soft tissue biomaterials.  
The pneumatic extrusion
nozzle type may have least or have no material leakage while the extrusion is paused or the  position is shifted.
The screw extruder nozzle type can produce high extrusion propulsion power, that is suitable for extrusion of high pressure tolerance and viscosity materials.

4. The precise temperature control:  Localized temperature control with accuracy of +/- 0.1
, ensuring a constant temperature and cell activity and can realize best spatial repeatability and process reproducibility of the bio-fabrication.

5. Precise controllable internal porosity and ways to print with multiple anglesensuring that the internal holes of the porous structure are 100% connected, providing similarity as in vivo cell growth microenvironment.  

6. Clean Design
The overall structure and materials of the 3D printing system are sterile design to ensure a clean working environment and processes, and are suitable for 3D printing of biological materials and cells under sterile conditions.  
Integrated supported professional sterile equipment, ensuring chance of contamination is less than 10%.  

7. Print Stage space
Large molding range, 160 x 160 x 150mm. with custom range available.

8. Display, editing and controls of a three-dimensional models
Implement features such as view, rotate, scale, move and mirror of 3D model, 3D model slicing, and motion stage and 3D model printing controls, etc. 
Free design with CAD softwares, have external shape and internal structure according to the model implementation requirements.

9. Integrated development teams
Integrated developed by Regenovo Biotechnology Co.Ltd. and Hangzhou University of Electronic Science and Technology, with completely independent intellectual property rights, and can implement partial customization according to specific user requirements to realize adjustable and diverse needs.  

10. Solid Living Cell Print foundation:  Professor Xu Mingen, our chief scientist and colleagues have dedicated in Cell-Printing for more than 8 years, and have prominent contributions in the bio-3D Print field. 

Generic Specifications
Air pressure: 0.6-0.8Mpa
Support plate: -5 ~ 65
Size & Weight: 64x50x70cm/50kg or 85x61x65cm/150kg
Data format: direct support for STL, GCODE, and customized proprietary format.
Operating System: Windows System
Power supply: 100-240V AC, 20A, 50/60Hz
Nozzle cleaning: with self-cleaning function
Customizable options
a.Lab & manufacturing process optimization.
Additional modules, as : Stereolithography (Light-Curing), Electrospinning, L
aser induced forward transfer modules,etc.

Cooperative partners

Based on progressive and open main frame, we have vigorous and intensive cooperations with our partners and power users in many advanced institutions, for instrument optimization, integrated process optimization, bio-material conditioning and tuning, to advanced bio-print and bio-fabrication projects and goals.
Part of our consitantly increasing partners:
Affiliated Hospital of People's Liberation Army General Hospital (304)

Brain Hospital of Tianjin, China Armed Police (Armed Forces Institute of traumatic brain injury and neurological disease)
Affiliated Drum Tower Hospital of Nanjing University
West China Hospital, Sichuan University, Research Center for Regenerative Medicine
117 Hospital of People's Liberation Army
Guangzhou Medical College
Stomatology Hospital of Zhejiang University (Biomedical Engineering and Instrument Science)
Hangzhou University of Electronic Science and Technology (Institute of Bio-medical engineering and instrumentation)
South China University of Technology (country rebuild human tissue engineering technology research center)
Shanghai Jiaotong University (State Key Laboratory of Metal Matrix Composites) ), etc...


We (Regenovo_ABDC) are a global technology and markiting partners, and cooperate seamlessly with research institutions, industry, universities, users for practical demands and successfully developed 3D-BioPrinting systems with independent intellectual properties and provide our users with flexible advantages, as expansion, modification, and innovative R&D applications.
Welcome to correspond for discussing your current demand and future developments.

The Regenovo is a high-tech enterprises specialized to provide integrated 3D Bio-Printing technology solutions in the biomedical field, and committed to the development of Bio-Printers bio-materials and software in the biomedical fields, with core research and development technologies.


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We (ABDC) have more than 20 years continuous efforts in the research and production of lab instrument, software and hardware, and have experiences on the 3D bio and food printing technology.

The Shining 3D Technology(Shining), is the largest 3D scanning and printing-related product development and distribution company, and also is one of the major technical partner of Regenovo.



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67. Patterned Hydrogel Substrates for Cell Culture with Electrohydrodynamic Jet Printing
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80. Chitosan—A versatile semi-synthetic polymer in biomedical applications
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82. A Printable Photopolymerizable Thermosensitive p(HPMAm-lactate)-PEG Hydrogel for Tissue Engineering
83. Three-Dimensional Fiber Deposition of Cell-Laden, Viable, Patterned Constructs for Bone Tissue Printing
84. Evaluation of Photocrosslinked Lutrol Hydrogel for Tissue Printing Applications
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97. A phase diagram for microfabrication of geometrically controlled hydrogel scaffolds
98. An Optical Method for Evaluation of Geometric Fidelity for Anatomically Shaped Tissue-Engineered Constructs
99. Printability of calcium phosphate powders for three-dimensional printing of tissue engineering scaffolds
100. Quantitative optimization of solid freeform deposition of aqueous hydrogels
101. Cell damage evaluation of thermal inkjet printed Chinese hamster ovary cells
102. Biocompatible Inkjet Printing Technique for Designed Seeding of Individual Living Cells
103. Three-dimensional inkjet biofabrication based on designed images
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107. Three-dimensional printing of stem cell-laden hydrogels submerged in a hydrophobic high-density fluid
108. Construction of 3D biological matrices using rapid prototyping technology
109. Cell Encapsulation in Biodegradable Hydrogels for Tissue Engineering Applications
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111. Improving Viability of Stem Cells During Syringe Needle Flow Through the Design of Hydrogel Cell Carriers
112. Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress
113. Fluid shear stress modulates surface expression of adhesion molecules by endothelial cells.
114. Delivery of human fibroblast cells by piezoelectric drop-on-demand inkjet printing
115. Biofabrication of Osteochondral Tissue Equivalents by Printing Topologically Defined, Cell-Laden Hydrogel Scaffolds
116. Shear-thinning hydrogels for biomedical applications
117. Rheological behavior of alginate solutions for biomanufacturing
118. Compression strength and deformation of gellan gels formed with mono- and divalent cations
119. Peptide-based stimuli-responsive biomaterials
120. Protein Engineering in the Development of Functional Hydrogels
121. Injectable PLGA based colloidal gels for zero-order dexamethasone release in cranial defects
122. Recent advances in the preparation of cyclodextrin-based polyrotaxanes and their applications to soft materials
123. Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering
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127. Protein release from alginate matrices
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129. Self-gelling hydrogels based on oppositely charged dextran microspheres
130. Biodegradable Colloidal Gels as Moldable Tissue Engineering Scaffolds
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132. Synthesis, Characterization, and Hydrolytic Degradation of PLA/PEO/PLA Triblock Copolymers with Short Poly(l-lactic acid) Chains
131. Influence of Amide versus Ester Linkages on the Properties of Eight-Armed PEG-PLA Star Block Copolymer Hydrogels
134. Polymer Networks Assembled by Host−Guest Inclusion between Adamantyl and β-Cyclodextrin Substituents on Poly(acrylic acid) in Aqueous Solution
135. Protein-Release Behavior of Self-Assembled PEG–β-Cyclodextrin/PEG–Cholesterol Hydrogels
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139. Stimuli-sensitive hydrogels: ideal carriers for chronobiology and chronotherapy
140. Synthesis and recovery characteristics of branched and grafted PNIPAAm–PEG hydrogels for the development of an injectable load-bearing nucleus pulposus replacement
141. Thermoresponsive hydrogels in biomedical applications
142. Reverse thermogelling biodegradable polymer aqueous solutions
143. Biodegradable Thermogels
144. Hydrogels for Protein Delivery
145. Photocrosslinkable Hyaluronan-Gelatin Hydrogels for Two-Step Bioprinting
146. Direct Freeform Fabrication of Seeded Hydrogels in Arbitrary Geometries
147. In-situ forming hydrogels by simultaneous thermal gelling and Michael addition reaction between methacrylate bearing thermosensitive triblock copolymers and thiolated hyaluronan
148. Click Chemistry for Drug Delivery Nanosystems
149. Enzyme-catalyzed crosslinkable hydrogels: Emerging strategies for tissue engineering
150. Hyaluronic Acid and Dextran-Based Semi-IPN Hydrogels as Biomaterials for Bioprinting
151. Replica multichannel polymer chips with a network of sacrificial channels sealed by adhesive printing method
152. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
153. Solvent bonding of poly(methyl methacrylate) microfluidic chip using phase-changing agar hydrogel as a sacrificial layer
154. Fabrication of microchannels using polycarbonates as sacrificial materials
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174. Dermal fibroblast infiltration of poly(ε-caprolactone) scaffolds fabricated by melt electrospinning in a direct writing mode
175. Direct Writing By Way of Melt Electrospinning
176. Tissue engineering: Perfusable vascular networks
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178. Variable cytocompatibility of six cell lines with photoinitiators used for polymerizing hydrogels and cell encapsulation
179. Some hydrogels having novel molecular structures
180. Super tough double network hydrogels and their application as biomaterials
181. Biodegradation of high-toughness double network hydrogels as potential materials for artificial cartilage
182. Mechanically strong hydrogels with reversible behaviour under cyclic compression with MPa loading
183. Why are double network hydrogels so tough?
184. Tissue assembly and organization: Developmental mechanisms in microfabricated tissues
185. Skin tissue generation by laser cell printing
186. Laser assisted bioprinting of engineered tissue with high cell density and microscale organization
187. Laser-assisted printing of alginate long tubes and annular constructs
188. Application of inkjet printing to tissue engineering
189. Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology
190. Tissue Engineering by Self-Assembly of Cells Printed into Topologically Defined Structures
191. Fabrication of soft tissue engineering scaffolds by means of rapid prototyping techniques
192. Scaffold-free vascular tissue engineering using bioprinting
193. Additive manufacturing for in situ repair of osteochondral defects
194. Multi‐nozzle deposition for construction of 3D biopolymer tissue scaffolds
195. Bioprinting of a mechanically enhanced three-dimensional dual cell-laden construct for osteochondral tissue engineering using a multi-head tissue/organ building system
196. Sodium Alginate Hydrogel-Based Bioprinting Using a Novel Multinozzle Bioprinting System
197. Characterization of printable cellular micro-fluidic channels for tissue engineering
198. Direct Construction of a Three-dimensional Structure with Cells and Hydrogel
199. Characterizing Environmental Factors that Impact the Viability of Tissue-Engineered Constructs Fabricated by a Direct-Write Bioassembly Tool
200. On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels
201. Generation of Three-Dimensional Hepatocyte/Gelatin Structures with Rapid Prototyping System
202. Three-dimensional Gelatin and Gelatin/Hyaluronan Hydrogel Structures for Traumatic Brain Injury
203. Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology
204. An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and agelatin/alginate/fibrinogen matrix
205. Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique
206. Rapid Prototyping Three-Dimensional Cell/Gelatin/Fibrinogen Constructs for Medical Regeneration
207. Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip
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210. Rheology of reconstituted type I collagen gel in confined compression
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216. Bioprintable, cell-laden silk fibroin–gelatin hydrogel supporting multilineage differentiation of stem cells for fabrication of three-dimensional tissue constructs, Acta Biomaterialia, 2015, 11, 233
217. Compartmentalized bioencapsulated liquefied 3D macro-construct by perfusion-based layer-by-layer technique, RSC Adv., 2015, 5, 4, 2511
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219. Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles,Biomaterials, 2015, 37, 174
220. Enhanced Pulsatile Drug Release from Injectable Magnetic Hydrogels with Embedded Thermosensitive Microgels, ACS Macro Letters, 2015, 312
221. Oxidation- and thermo-responsive poly(N-isopropylacrylamide-co-2-hydroxyethyl acrylate) hydrogels cross-linked via diselenides for controlled drug delivery, RSC Adv., 2015, 5, 6, 4162
222. Peptide modification of purified gellan gum, J. Mater. Chem. B, 2015, 3, 6, 1106
223. Rapid Formation of a Supramolecular Polypeptide-DNA Hydrogel for In Situ Three-Dimensional Multilayer Bioprinting, Angewandte Chemie, 2015, n/a
224. Rapid Formation of a Supramolecular Polypeptide-DNA Hydrogel for In Situ Three-Dimensional Multilayer Bioprinting, Angewandte Chemie International Edition, 2015, n/a
225. Use of the polycation polyethyleneimine to improve the physical properties of alginate–hyaluronic acid hydrogel during fabrication of tissue repair scaffolds, Journal of Biomaterials Science, Polymer Edition, 2015, 1
226. 3-dimensional (3D) fabricated polymer based drug delivery systems, Journal of Controlled Release, 2014, 193, 27
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228. A Novel Poly(amido amine)-Dendrimer-Based Hydrogel as a Mimic for the Extracellular Matrix, Advanced Materials, 2014, 26, 24, 4163
229. Biofabrication of tissue constructs by 3D bioprinting of cell-laden microcarriers, Biofabrication, 2014, 6, 3, 035020 hierarchical scaffolds consisting of micro-sized 230. polycaprolactone (PCL) and electrospun PCL nanofibers/cell-laden alginate struts for tissue regeneration, J. Mater. Chem. B, 2014, 2, 3, 314
231. Construction of cellulose–phosphor hybrid hydrogels and their application for bioimaging, J. Mater. Chem. B, 2014, 2, 43, 7559
232. Covalent attachment of a three-dimensionally printed thermoplast to a gelatin hydrogel for mechanically enhanced cartilage constructs, Acta Biomaterialia, 2014, 10, 6, 2602
233. Development and characterisation of a new bioink for additive tissue manufacturing, Journal of Materials Chemistry B, 2014, 2, 16, 2282
234. Enzymatic synthesis of hyaluronic acid vinyl esters for two-photon microfabrication of biocompatible and biodegradable hydrogel constructs, Polym. Chem., 2014, 5, 22, 6523
235. Externally addressable hydrogel nanocomposites for biomedical applications, Current Opinion in Chemical Engineering, 2014, 4, 1
236. Highly Conductive and Flexible Silver Nanowire-Based Microelectrodes on Biocompatible Hydrogel, ACS Applied Materials & Interfaces, 2014, 6, 21, 18401
237. Hydrogels in a historical perspective: From simple networks to smart materials, Journal of Controlled Release, 2014, 190, 254
238. Hydrogels to model 3D in vitro microenvironment of tumor vascularization, Advanced Drug Delivery Reviews, 2014, 79-80, 19
239. Laser Photofabrication of Cell-Containing Hydrogel Constructs, Langmuir, 2014, 30, 13, 3787
240. Melt Electrospinning and Its Technologization in Tissue Engineering, Tissue Engineering Part B: Reviews, 2014, 150127064140006
241. New Methods in Tissue Engineering: Improved Models for Viral Infection, Annual Review of Virology, 2014, 1, 1, 475
242. Nucleobase peptide amphiphiles, Materials Horizons, 2014, 1, 3, 348
243. Properties of Polylactide Inks for Solvent-Cast Printing of Three-Dimensional Freeform Microstructures, Langmuir, 2014, 30, 4, 1142
244. Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model, Journal of The Royal Society Interface, 2014, 11, 98, 20140501
245. Reactive macromolecular micelle crosslinked highly elastic hydrogel with water-triggered shape-memory behaviour, Polymer Chemistry, 2014, 5, 17, 4965
246. Strain hardening and highly resilient hydrogels crosslinked by chain-extended reactive pseudo-polyrotaxane, RSC Adv., 2014, 4, 100, 56791
247. Synthesis and High-Throughput Processing of Polymeric Hydrogels for 3D Cell Culture, Bioconjugate Chemistry, 2014, 25, 9, 1581
248. The Design of Dextran-Based Hypoxia-Inducible Hydrogels via In Situ Oxygen-Consuming Reaction, Macromolecular Rapid Communications, 2014, 35, 22, 1968
249. Scaffold Design and Fabrication
150. Tough Stimuli-Responsive Supramolecular Hydrogels with Hydrogen-Bonding Network Junctions, Journal of the American Chemical Society, 2014, 136, 19, 6969
251. Directed assembly of cell-laden hydrogels for engineering functional tissues
252. Self-assembly and tissue fusion of toroid-shaped minimal building units
253. Encapsulated arrays of self-assembled microtissues: an alternative to spherical microcapsules

254. Generation and Assessment of Functional Biomaterial Scaffolds for Applications in Cardiovascular Tissue Engineering and Regenerative Medicine Svenja Hinderer, Eva Brauchle, and Katja Schenke-Layland Advanced Healthcare Materials (2015) : n/a

255. Tissue vascularization through 3D printing: Will technology bring us flow?
S.J. Paulsen and J.S. Miller Developmental Dynamics (2015) 244: 629
256. The potential of induced pluripotent stem cells in models of neurological disorders: implications on future therapy Jeremy Micah Crook, Gordon Wallace, and Eva Tomaskovic-Crook Expert Review of Neurotherapeutics (2015) 15: 295
257. Bioprinting Is Changing Regenerative Medicine Forever Scott Forrest Collins Stem Cells and Development (2014) 23: 79
258. Organogenesis special issue – preface, Developmental Dynamics, 2015, 244, 3

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