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Engineered Cells for Science and Pharma
   
Bio-Technology Raw Materials and Consumables from Taiwan
Knowledge Bases
Cell Line, Immortalization, Transgene, RMCE,
GOI, Vector, CHO, HEK293, GPCR, Cell Culture, Drug Development, Personalized, Medicine, Characterization phenotype, GMO, ECACC, Heterologous expression,
Receptor, Ion Channel, High Throughput Screening, Protein Production, Antigen Presentation,
Primary cells / tissues, Medical model / Disease model, Patient group, Bioassays, 3D cell culture, Preclinical development / Preclinical research
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Expansion of Functional Personalized Cells with specific Transgene combinations
Bilingual Translation by ABDC (ABDC的雙語翻譯, By Lky)
https://lnkd.in/ggimMmdc
Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development. |
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Stable CHO GPCR cell line
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. Stable G-protein coupled receptor (GPCR)
. CHO cell lines for cell-based assays.
. Available as single seed vials or as frozen, assay ready reagent.
. Available via the European Collection of Authenticated Cell Cultures (ECACC).
. All main GPCR subunits available (Gs, Gq/G11, Gi/G0).
. Cell lines are published.
. Clonally-derived, GMO class 1
https://www.inscreenex.de/products/gpcr-cell-lines/ |
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Receptor
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Cell Line (ECACC Cat. No.)
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GPCRdb
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ADRB2 (ADRB2R, B2AR)
β2-adrenoceptor, beta-2 adrenergic receptor
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10031601
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Link
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CHRM1
Muscarinic acetylcholine receptor M1
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10110203
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Link
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CHRM2
Muscarinic acetylcholine receptor M2
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10110204
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Link
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CHRM3
Muscarinic acetylcholine receptor M3
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10031603
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Link
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CHRM5
Muscarinic acetylcholine receptor M5
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11041302
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Link
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HRH2
Histamine H2 receptor, H2R, HH2R, Gastric receptor I
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10031604
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Link
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FFAR2 (FFA2, GPCR43, GPR43)
Free fatty acid receptor 2, G-protein coupled receptor 43
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10110202
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Link
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FFAR3 (GPR41)
Free fatty acid receptor 3, G-protein coupled receptor 41
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10031602
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Link
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FFAR4 (GPR120, GPR129, O3FAR1, PGR4)
Free fatty acid receptor 4, G-protein coupled receptor 120, G-protein coupled receptor 129, G-protein coupled receptor GT01, G-protein coupled receptor PGR4, Omega-3 fatty acid receptor 1
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10110205
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Link
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OPRL1 (OOR, ORL1, NOPR) Nociceptin receptor, Kappa-type 3 opioid receptor, KOR-3, Orphanin FQ receptor
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10110201
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Link
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SSTR1
Somatostatin receptor type 1, SS-1-R, SS1-R, SS1R, SRIF-2
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10110206
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Link
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GCGR
Glucagon receptor, GL-R
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10031605
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Link
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Heterologous expression of the target of your choice#Receptor, #Ion Channel, #YOUR TARGET
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Your cells for your application#High Troughput Screening, #Protein Production, #Antigen Presentation, #YOUR APPLICATION
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Immortalized Cell Lines
Species : Human, Mouse, Chicken, Sheep, Pig/Swine, Llama/Camelid, Cow/Cattle, Dog, Cat, Rat
Cell Types
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1. Epithelial Cells
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2. Endothelial Cells
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3. Stromal Cells
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4. CNS/PNS/ENS Cells
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5. Musculoskeletal cells
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Alveolar Epithelial Cells
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Microvascular Endothelial Cells
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Fibroblasts (skin, gingiva)
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Enteric Glial Cells
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Chondrocytes
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Airway Epithelial Cells
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HUVECs
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MSCs
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Astrocytes
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Osteoblasts
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Thyroid Epithelial Cells
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Lymphatic Endothelial Cells
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Pericytes
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Spinal Cord Neurons
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Smooth Muscle Cells
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Intestinal Epithelial Cells
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Aortic Endothelial Cells
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Wharton's Jelly MSCs
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Hepatocytes
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Cardiac Stromal Cells
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Placenta Epithelial Cells
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Kidney PTECs
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Retinal Epithelial Cells
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Nasal Epithelial Cells
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Keratinocytes
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Bronchial Epithelial Cells
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Mammary Epithelial Cells
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Cell Culture Media and Supplements
Optimized Cell Culture Media ensuring growth and maintenance of cellular properties, consisting of Basal Medium and corresponding Supplements.
Culture media :
huAEC Medium, huAEC Medium, huChon Medium, Serum-Free huChon Medium, huFIB Medium, Serum-Free huFIB Medium, huMEC Medium, huOB Differentiation Medium, huOB Maintenance, Medium, huVEC Medium, huThyrEC Medium, muINTEPI Medium, muADINTESTI Medium, muMEC Medium
Coating solutions :
Collagen solution (ready-to-use), from rat tails, suitable for cell culture
Collagen solution (ready-to-use), from rat tails, suitable for cell culture
Gelatin solution 2% (ready-to-use), suitable for cell culture
Gelatin solution 2% (ready-to-use), suitable for cell culture
huAEC Coating Solution (ready-to-use), suitable for cell culture
huAEC Coating Solution (ready-to-use), suitable for cell culture
huAEC Coating Solution (ready-to-use), suitable for cell culture
Epithelial Cell Coating Solution (ready-to-use), suitable for cell culture
Epithelial Cell Coating Solution (ready-to-use), suitable for cell culture
Various :
Doxycycline solution (2 mg/ml), suitable for cell culture
Freezing medium (contains serum)
G418 solution (50 mg/ml), suitable for cell culture
Polybrene solution (8 μg/ml), suitable for cell culture.
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