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FaEChERS
The
"Fast, Easy, Cheap, Effective, Rugged, and Safe(FaEChERS)"
Sample Extraction Kit & Method for Pesticide Residues
Determination
How to shorten the time required of chemical determination
for the pesticide residues in agricultural products, have been a global common
issues,
among which the extraction of pesticide residues is the most time-consuming
step.
A rapid chemical extraction technology developed by our cooperative team,
the FaEChERS is a
collection of much more faster and more advanced
kit and method for
extractions,
that can significantly reduce more than 85% time
for the
extraction of pesticide residues in fruits and vegetables.
In spite that
the current most widely & globally applied technology
for extraction of pesticide residues in fruits and vegetables is the
QuEChERS
("Quick, Easy, Cheap, Effective, Rugged, and Safe"),
the sample extraction steps
and time
of our
FaEChERS
technology is
significantly reduced
to only 1/30
of
QuEChERS
extraction time,
that effectively enhances the timeliness of pesticide residue testing.
Therefore, FaEChERS
technology has the very high potential
to replace the QuEChERS.
We hope to be able to draw on technical and marketing capacity of international
companies,
for quick and
effective international promotions,
including the progressive striving to become the standard methods
in the AOAC, EU CFDA, etc., and to will maximize application values and provide
important helps
to the
international agricultural and analytical fields.
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Comparison
of the extraction steps and time required for FaEChERS and
QuEChERS :
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QuEChERS
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FaEChERS
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1. the solvent
is added to the sample.
2. add
extraction powder with short shaking.
3. drastic shaking
4. centrifuge
5. retrieve the
supernatant
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1. Add the solvent to the sample with brief shaking and filtration.
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6. Add
purification powder
7. drastic shaking once again
8. centrifuged again
9. supernatant
10. add to
volume
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10 total steps,
30 minutes
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One step, 1
minute.
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FaEChERS patents has been filed:
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2014, ROC patent
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2014, People's Republic of China patent
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2014, US patent
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More
patents pending, ....
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FaEChERS technical features:
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1. One step to complete the extraction of pesticide residues
in sample.
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2. Save more than 85% extraction time required for pesticide
residues.
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3. Improve the efficiency of pesticide residues extraction
about five times.
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4. Reduce solvent use by about 50%.
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5. Save 60% labor costs for the pesticide residue
determinations.
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Comparison
of
the
performance
of
extraction kit
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Item
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Traditional SPE
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QuEChERS
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FaEChERS
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Steps required
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<= 20 steps
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>= 10 steps
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1-2 steps
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Time required
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60 minutes to 48 hours
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30 minutes
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1 minutes
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Accuracy
(recovery)
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60% -140%
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60% -140%
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60% -140%
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Precision
(coefficient of variation)
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< 20
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< 20
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< 20
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Limit of
detection
(LOQ)
<= 0.01ppm
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251 pesticides,
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310 pesticides
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355-408 pesticides
(depending on crops)
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Linearity
(correlation coefficient)
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> 0.99
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> 0.99
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> 0.99
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Matrix Effects
Assessment
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High matrix effects
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Lower matrix effects
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Much lower matrix effects
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The validation of FaEChERS extraction kits
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Recovery(%)
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mango
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shallots
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greens
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apples
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carrots
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avocado
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pea
sprouts
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<60
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57
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63
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61
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53
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40
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42
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35
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60-140
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355
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351
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353
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363
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371
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401
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408
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> 140
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6
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4
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6
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4
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9
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1
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1
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Doses
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418
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418
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420
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420
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420
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444
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444
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Mango, shallots, greens, apples, carrots, avocado, pea sprouts matrix used:
In the 60% -140% recovery range, there are 355, 351,
353, 363,
371, 401 and 408 items, that respectively account for
85%, 84%, 86%, 88%, 90% and 92% of the total number of pesticides.
The range collectively account up to 94% and 98.6% of the total number
of pesticides
that are ever and
frequently detected respectively.
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The pesticides items with the Limit of detection(LOQ) available to 0.01ppm,
for Mango matched with reagents and methods of FaEChERS, for example,
is up to
388 items, accounting for
92.8% of the total pesticides analyzed.
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LOQ(ppm)
range(<=)
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Items
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<=0.01
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388
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>0.01 <=0.025
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15
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>0.025 <=0.05
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8
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>0.05 <=0.1
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2
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>0.1 <=0.25
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4
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>0.25 <=0.5
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0
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>0.5, <=1
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1
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