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High Performance Microspheres --
Chromatography and other applications
Informations List
Broad application
spectrum :
Our High Performance Microspheres applications have
spanned a broad spectrum,
not limited to but including:
High performance chromatography media,
Solid phase extraction (SPE) sorbents,
Metal scavenger resins,
Particle size standards,
Magnetic particles,
Fluorescent microspheres,
Enzyme support,
Drug carriers,
LCD spacer,
ACF conductive particles, and various other
functional microparticles.
New applications for microspheres are discovered every day,
below are just a few:
(https://en.wikipedia.org/wiki/Microsphere#Applications)
Assay - Coated microspheres provide measuring tool in
biology and drug research
Buoyancy - Hollow microspheres are used to decrease
material density in plastics(glass and polymer)
Particle image velocimetry - Solid microspheres used
for flow visualization, typically with density
matching that of the
fluid.[5]
Ceramics - Used to create porous ceramics used for
filters (microspheres melt out during firing,
Polyethylene
• Microspheres) or used to prepare highstrength lightweight
cocnrete.[6]
Cosmetics - Opaque microspheres used to hide wrinkles
and give color, Clear microspheres provide
"smooth ball bearing" texture during
application (Polyethylene Microspheres)
Deconvolution - Small fluorescent microspheres (<200
nanometers) are required to obtain
an experimental Point spread function to characterise microscopes and perform
image deconvolution.
Drug delivery - As miniature time release drug capsule
made of, for example, polymers. A similar use is
as outer shells of microbubble
contrast agents used in contrast-enhanced ultrasound.
Electronic paper - Dual Functional microspheres used in
Gyricon electronic paper
Insulation – expandable polymer microspheres are used
for thermal insulation and sound dampening.
Personal Care - Added to Scrubs as an exfoliating agent
(Polyethylene Microspheres)
Spacers - Used in LCD screens to provide a precision
spacing between glass panels (glass)
Standards - monodispere microspheres are used to
calibrate particle sieves,
and particle counting apparatus.
Retroreflective - added on top of paint used on roads
and signs to increase night visibility
of road stripes and signs (glass)
Thickening Agent - Added to paints and epoxies to
modify viscosity and buoyancy
Drugs can be formulated as HBS floating microsphere.
Followings are list of drugs which can formulated as
microsphere: Repaglinide, Cimetidine,
Rosiglitazone, Nitrendipine, Acyclovir,
Ranitidine HCl, Misoprostol, Metformin,
Aceclofenac, Diltiazem, L-Dopa and
beneseragide, Fluorouracil [4]
The Nano-Micro
Tech(abreviated as NMT) is devoted to developing a world-leading
technology for providing the highest quality and the largest selection of
uniformly sized nano- and microspheres (microparticles).
http://www.nanomicrotech.com/
The company is capable of supplying monodisperse particles of almost any size
ranging from 5 nanometers to 1000 microns of different materials,structures, and functionalities.
More than 10 current product lines with over 5000 products which continue to
evolve and expand to serve rapidly growing industries of cutting edge
technologies.
We(ABDC) and NMT,
(abreviated as NMT_ABDC) are global technology and sales partners,
serving application commitments for users in research institutions, industry
sections, universities, and have successfully developed high performance
microspheres with globalized intellectual properties.
http://www.chromnet.net/
The leading
manufacture source:
We are one of the few suppliers that are capable of
globally providing monodisperse
microsphere species:
Monodisperse silica gel chromatography
packing (globally exclusive production)
Homogenic polymeric particle bioseparation
media (global second source)
Liquid crystal display(LCD) SPACER,
conductive beads, light-diffusing microspheres
Standard particles, magnetic beads,
fluorescent microspheres
Enzyme carrier, drug carriers.
Experiences and
achievements :
Outstanding research and production
experiences and achievements:
The Company founder, Dr. Jiang Profile:
1984-1988 Bachelor of Chemistry
Department of Chemistry, Peking University.
1994-1998 doctorate of SUNY Binghamton University
1998-2000 postdoctoral of University of California at Berkeley
2000-2006 Senior researcher, Rohm and Haas Company
2006- to date Chief Scientist, Shenzhen Micro Technology Co., Ltd.
2007- to date Professor/Director, Peking University Shenzhen Graduate School
Center for Nanoscience.
2008- to date Chairman/Chief Scientist, Suzhou Micro
Technology Co., Ltd.
Dr. Jiang is eligible for State 1,000 plans, Jiangsu Province entrepreneurship
and innovation talent, entrepreneurship and innovation talent Suzhou, first
leading talents for Suzhou Industrial Park's.
Dr. Jiang is satisfied Professor/Director of Nano-Micro Materials Research
Center, of Peking University Shenzhen Graduate School .More than 30 patents applied in country
and abroad, and
published 28 articles on
prestigious journals. The research result has been widely used in academic
research and industry sectors.
NMT build a nano-micron material research center with the Peking University
Shenzhen Graduate School, the center was founded in 2007 as a key Laboratory of
Guangdong Province.
Our Development Goals:
Liquid crystal Display(LCD) spacer
Light-diffusing beads
Conductive golden
sphere
Separation and purification media for biopharmaceutical drug
Drug release control carrier
Enzyme immobilization carrier
Drug testing and analysis
Wastewater purification
LED lighting
Heavy metal recycling
Environmental Monitoring
Energy saving
Core technology
platform:
Preparation
of monodisperse microspheres in any sizes between 5 nanometers to 1000
micrometers.
Control of microsphere structures, such
as specific surface area, pore size and
distribution.
Microsphere surface functionalization
technology
Preparation of the special structure and
multi-functions of nano-micron composite
microspheres
Scaled microsphere production and
application technology.
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Size and
distribution
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of the microspheres |
Microsphere
morphology |
Substrate and
surface
characteristics |
Scanning electron micrographs of Submicron
Spheres
Scanning electron micrographs of Micro
Spheres
Optical electron micrographs of Micro
Spheres
Standard particles
products:
NMT has more than
100 polystyrene standard particles, and is one of the blobal providers that can
offer most standard particles.
The particle size range from 50 nanometers to 1000 micrometers. |
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SEM Picture |
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High-performance LCD SPACER:
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LCD SPACER is the key
microspheres materials that is placed between two glass substrates in a liquid
crystal display, for controlling the thickness of liquid crystal layer.
NMT is a company after Japan's Sekisui & Hayakawa Rubber
that are satisfied to produce
spacer products.
Our current sales has been ranked first in the world.
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Magnetic Nano
Microspheres:
SEM and TEM images of all kinds of magnetic
nano microspheres from NMT:
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Nanoscale magnetic
particles |
Submicron magnetic
particles |
_1.jpg)
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Magnetic Field
(Oe) |
Micron magnetic
particles |
Standard Bioprocess:
Bio
Manufacturer-Bacteriai/Animal Cell:
Bioseparation:
Chromatography
Process (1):
_1.jpg)
Chromatography Process(2):
_1.jpg)
Chromatographic Terms:
Separation Mechanism:
Types of NMT
chromatography packings:
Factors of separation medium:
The main factors that determine the
performance of the separation medium:
ØMicrosphere Matrix chemical composition
and physical properties
ØMicrosphere Size and particle size
uniformity
ØMicrosphere morphology, pore size
distribution and pore volume, specific surface area
ØMicrosphere surface functional groups and
density
ØMicrospheres of mechanical properties and
chemical stability, hardness, etc.
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Size and
distribution
of the microspheres |
Microsphere
morphology |
Substrate and
surface
characteristics |
Chromatography silica gel:
Unique monodisperse silica gel
chromatography packing:
The NMT has worldwide exclusively produced full range of monodisperse silica gel
chromatography packing.
We can roduce mono-dispersive, porous silica gel for chromatography packing, at
any size ranging from 1.7 to 50 μm,
with routine pore sizes 100, 120, 170, 300, 500 Å.
Surface Bonding & End-capping:
Innovative silica gel surface
bonding and end-capping technology:
The NMT not only has the worldwide exclusive monodisperse porous silica gel
production technology, but also developed innovative the surface bonding and
endcapping technology,
enabling high selectivity, high-resolution, and high chemical stability with the
produced silica gel packing.
Silica gel
comparison:
Comparison of NMT silica gel packing with well
known foreign brands:
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NMT has developed ultra-pure silica gel
packings for the analytical, semi-preparative and scaled production needs.
Our packings is featured with precise particle size, high particle size
uniformity(CV <3%), extreme high purity(> 99.99%),
high surface area ratio and optimized pore size.
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Comparison of particle size distribution |
Repeatability & Stability:
Repeatability and chemical stability of scaled products:
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Acid and base resistance |
Column: UniSil™ 10 C18, 4.6*250mm
Eluent: 0.1 mol/L NaOH/CH3OH=40/60 for base
0.2 mol/L CH3COOH/CH3OH =50/50 formic acid
Test of column efficiency: Sample:Toluene
Mobile phase: ACN/H2O=60/40
Flow rate: 1.0 ml/min,
Detection: UV@254nm |
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Repeatability between batches |
Chromatography performance
Particle size and size distribution
Pore size and surface area
Bonded phase density |
High resolution and
high selectivity:
Silica gel series:
Full range of high performance silica gel
chromatography packing:
Complete &
Monodisperse
The world's most complete monodisperse polymer
chromatography packing:
After GE, the NMT is the second company
capable to scaled production of chromatography packing with uniform particle
polymer,
Our product range has surpassed GE, with most complete monodisperse polymer
chromatography packing.
The routine particle size are 1.7, 3, 5, 10, 20, 30, 40, 50 microns,
with optional pore sizes of 100, 300, 500, 800, 1000Å.
Uniform
particle & pore sizes
Chromatography packing from uniform polymer particle with various pore sizes:
Different substrates:
Polymer packings with different substrate composition:
Large-scale reproduciblity:
Very high reproduciblity between large-scale
batch productions
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Our “state-of-the-art” manufacturing facilities, advanced
analytical
instrument, and reliable
quality
control system guarantee the
high quality and batch to batch reproducibility of
products. |
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Batch reproducible:
Highly reproducible from batch to batch:
Resistance:
Excellent acid and base resistance performance:
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Breakthrough
Curve
for Insulin.
The insulin
capacity was
well
maintained even
after 10 um
(NM-UNI-PS10-RPC-300)
media was
exposed to 1M NaOH aqueous solution at 60℃ for 40 days.
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Alkali resistance
test for Polymer packing |
Most complete
packing:
Most complete monodisperse polymer chromatography
packing:
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NMT
offers the most complete uniform particle polymer
chromatography packing!
Packings for ultra-high pressure, semi-preparative, and to industrial
preparative chromatography, to meet the customer needs.
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Advantages:
Advantages of the NMT monodisperse polymer chromatography
packings:
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lEasily packed, samll back pressure, quick
flow speed
lLarge surface area, greater adsorption amount
lConcentrated elution, less elution volume
lGood Selectivity, higher recovery
lWider applicable pH(1-14) range
lExcellent chemical stability, in-place cleaning
and sterilization
lLonger durability |
Why Uniform Particle?
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The more uniform
of packing particle, the narrower the peak shape, with higher column efficiency
and higher separation efficiency. |
Silica gel vs. Polymer packing:
lpolymer and silicone filler has a complementary choice in
separation
lpolymer has
strong resistance to acid and base, and with long duribilities.
lpolymer packings are easy to regenerate and clean, good batch
to batch reproducibility.
lsilica gels has high mechanical strength, high column
efficiency, is easily packed.
lsilicone packings has high-resolution.
Ion chromatography:
Uniform particle ion chromatography packings:
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The high efficiency uniform particle ion
chromatography packings is synthesize by our unique patented technology, with
characteristics as large loading capacity, high resolution, good selectivity,
excellent acid/base resistance, among others. |
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High-performance:
High-performance ion-exchange chromatography
packing:
lIncreased Loading Capacity
lUnmatched Rigidity
lImproved Chromatographic
Resolution
lConsistent Lot-to-lot Reproducibility
High efficient bio-analysis and separation:
Hydrophobic
chromatography packing:
We provides a series of hydrophobic chromatography packings that can be used
for analysis and separation of biomolecules.
Efficient separation and purification of
proteins
Nickel affinity
chromatography packing:
We has developed a series of Ni affinity chromatography packings that
efficiently isolate proteins.
Carbohydrate Chromatographic Media
NMT has developed a complete line of high performance chromatographic media
for the analysis and separation of carbohydrates. The media are prepared by
sulfonating poly (styrene-co- divinylbenzene) microspheres
followed by chelating of various metal ions such as calcium (Ca2 +) and sodium
(Na +).
Solid phase extraction packing:
NMT uniform particle solid phase extraction packing
Chromatography
packings and columns:
Prepacked columns:
Total technical solutions:
We provide customer total technical solutions:
lThe most complete
high-performance liquid chromatography packings, including normal phase, reverse
phase, ion exchange, size exclusion, hydrophobic interaction and affinity
chromatography packings, that is composed respectively based on three
substrates, ie. silica gel, crosslinked polystyrene and polymethyl methacrylate
esters, which is in performance complementary.
lAccumulations of large number of micro-separation
and purification expertise, and quick, economical separation and purification
process, providing customers some or all of their separation and purification
solutions.
lHigh-quality products and optimization scheme for
separation and purification to reduce production costs, accelerate the
industrialization of products, and reduce the use of solvents.
Cases & examples:
Successful examples in isolation and purification:
lSeparation and purification method in
traditional Chinese medicine and plant active ingredient, including complete
separation and purification schemes from small scale to pilot scale and then
full scale. (Success Story: Breviscapine; paclitaxel; alkali huperzine A;
resveratrol; polyphenols; stevioside; Ginkgolides ....)
lExtraction of bio-pharmaceutical, separation,
purification methods, with complete preparation system and technique solutions.
(Success Story: insulin; thymalfasin and the like; vancomycin enzyme and its
derivatives; daptomycin; Interferon; Thymopentin; cyclic peptide; teicoplanin;
trypsin; NADP ...).
lPolysaccharide, isolation, purification method
(Success Story: lentinan; stevioside; ...)
lOther products, separation and purification
technique, and complete preparation system and technique solutions.(Success
Story: If fondaparinux sodium Gan Kui; fullerenes ....)
lDetection of complex systems, such as food safety
testing, pesticide residues, etc., to provide the best HPLC and SPE columns and
other consumables.
Linear velocity:
Typical practice in calculation of linear velocity
of mobile phase:
Packing evaluation(1) - number of
theoretical plates
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Packing evaluation(2) - peak symmetry
_1.jpg)
Resolution: selectivity vs. column
efficiency
Resolution calculation
Salt
effect:
Effect of salt concentration on resolutions:
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