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Agarose/#Protein_purification
https://en.wikipedia.org/wiki/Agarose
https://en.wikipedia.org/wiki/Agarose#Protein_purification

An agarose is a polysaccharide polymer material, generally extracted from seaweed. Agarose is a linear polymer made up of the repeating unit of agarobiose, which is a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose.[1] Agarose is one of the two principal components of agar, and is purified from agar by removing agar's other component, agaropectin.[2]

Agarose is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the warm, liquid solution into a mold. A wide range of different agaroses of varying molecular weights and properties are commercially available for this purpose. Agarose may also be formed into beads and used in a number of chromatographic methods for protein purification.


An agarose gel in tray used for gel electrophoresis

Applications: Protein purification[edit]
Agarose gel matrix is often used for protein purification, for example, in column-based preparative scale separation as in gel filtration chromatography, affinity chromatography and ion exchange chromatography. It is however not used as a continuous gel, rather it is formed into porous beads or resins of varying fineness.[16] The beads are highly porous so that protein may flow freely through the beads. These agarose-based beads are generally soft and easily crushed, so they should be used under gravity-flow, low-speed centrifugation, or low-pressure procedures.[17] The strength of the resins can be improved by increased cross-linking and chemical hardening of the agarose resins, however such changes may also result in a lower binding capacity for protein in some separation procedures such as affinity chromatography.
Agarose-based gel filtration columns used for protein purification on an AKTA FPLC machine.

Agarose is a useful material for chromatography because it does not absorb biomolecules to any significant extent, has good flow properties, and can tolerate extremes of pH and ionic strength as well as high concentration of denaturants such as 8M urea or 6M guanidine HCl.[18] Examples of agarose-based matrix for gel filtration chromatography are Sepharose and WorkBeads 40 SEC (cross-linked beaded agarose), Praesto and Superose (highly cross-linked beaded agaroses), and Superdex (dextran covalently linked to agarose).

For affinity chromatography, beaded agarose is the most commonly used matrix resin for the attachment of the ligands that bind protein.[19] The ligands are linked covalently through a spacer to activated hydroxyl groups of agarose bead polymer. Proteins of interest can then be selectively bound to the ligands to separate them from other proteins, after which it can be eluted. The agarose beads used are typically of 4% and 6% densities with a high binding capacity for protein.

Agarose is proven to be excellently compatible with natural biomolecules like proteins, DNA carbohydrates etc.
The material shows minimal non specific interaction due to hydrophilic nature of agarose.
Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the microenvironment in the column and distorted separations.

Advanced Protein purification system
http://www.chromnet.net/Downstream Processing Solution_English.aspx

Leading with Quality, Performance and Cost :
Our partners, the Bio-Works started 2006 is one top Professional Agarose based chromatography media innovator experienced in the biotech industry and in protein purification.
Located in Sweden with quality management system (QMS) based on standards of ISO 9001:2008,our partners has leverages quality documents and support on request, technical support, certificates, statements, vendor audits and regulatory support information.
Bio-Works produces agarose based high performance products in industrial scale for uses in research and production within areas of Research & Laboratory, Life Science & Biopharma and Food & Beverage.

http://www.bio-works.com/

Intellectual Property and Know How
Bio-Works has patents covering improved agarose bead production methods, with know how and intellectual property to produce high rigidity agarose-based beads that is very important to avoid compression under high flow rates, allowing very high flow rates and large loading fluid be processed economically.  

Research Bioprocess Food & Beverage
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