Preparative Chromatography Systems DAC/M/HPLC/SAC/Gels |
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CPhI China 2016 & Circumstance
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CPhI China 2015 & Circumstance
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2016 Southwest Region Biopharmaceuticals separation and purification technology
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Leading with Quality, Performance and Cost :
We(Gelai_ABDC) are a global technology and
marketing team, with continuous efforts for more than 20 years ,
cooperating
seamlessly with all users in research institutions, industry, universities, for
their practical demands, and successfully developed DAC M/HPLC systems with
independent intellectual properties.
http://www.cdgelai.com/
http://www.chromnet.net/
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1. More than >500 large
& Total > 2100 systems
installed, with abundant high throughput,
high efficiency large-scale DAC-HPLC experiences for more
than 15 years. |
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2. Dedicated optimized Dynamic Axial
Compression Column(DAC Column) design,
approaching high resolution from the analytical HPLC
column. |
3. Middle/High pressure Gradient Pump(M/HPLC
Pumps) capability,
with
precision screw module that promise very stable & precise mobile phase output. |
4. 1/2 to 2/3 cost, very helpful to
optimized budgets controls, especially for cost management for acquiring more
equipment. |
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Product catalogue
User Manual_Preparative_HPLCPump_GLP3000-500ml_ChineseToEnglish.pdf
User Manual_UV Spectrophotometric Detector_UV-3292_ChineseToEnglish.pdf
User Manual_FractionCollector_GL-LF096_ChineseToEnglish.pdf
User Manual_ChromatographyWorkStation_ChineseToEnglish.pdf
User Manual_SAC_Column_ChineseToEnglish.pdf
User Manual_SAC500 ChromatographyColumnSystem_ChineseToEnglish.pdf
System Design Qualification &
Verification(DQ,IQ,OQ,PQ) :
Please contact for specific system configurations.
(Click
image for large images)mage for large images)
The Pump and Detector Assembling:
The DAC M/HPLC retains and scale-up the very
mature and stable separation technologies of the analytical High Performance
Liquid chromatography(HPLC) system, and has become the predominate purification
preparation methods with highest separation power and resolutions in many
fields, as biochemistry, biotechnology, pharmaceuticals, natural products,
organic synthesis, fine chemicals, preparation of standards, and food
ingredients fields, etc.
Factory Facility:
System Modules:
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Diameter ID25.4mm, 41.4mm, 50mm, 77mm, 80mm,
100mm, unique semi-preparative/preparatory chromatography columns(prePacked
Column / SAC Column),
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DAC-ID100mm, 150mm, 200mm, 400mm, 600m,
800m, 1000,1200mm, the Dynamic Axial Compression column systems(DAC Column)
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GLP-100ml, 250ml, 500ml, 1000ml, 2000ml,
3000ml, 5000ml Preparative High-Pressure Gradient Solvent Delivering Pump
systems(M/HPLC Pumps)
and other ancillary products, forming a
complete set of chromatography systems from laboratory to mass
preparative
productions.
System Accuracy &
Optimizations:
Our Various types of accurate middle to high pressure Solvent Delivering
Pumps(M/HPLC Pumps), can respectively withstand nearly 6000-3000psi, with
extremely small flowing pulse <= 1Bar(0.1Mpa),
which means that the users can convert and scale-up most of the separation
conditions
form analytical HPLC to our preparative M/HPLC systems with DAC, SAC or pre-Packed columns.
Our DAC/SAC Columns is optimally designed with
patented Forced Fluid Path Distributing technology
both for the column inlet and outlet, making more uniform and stable fluid path
distributing.
As the most helpful technology and business
partner for advanced technology manufacturers and laboratories,
We offer the ability to closely integrate customized developments.
We develope and manufacture preparative
HPLC/MPLC systems with same or better functionality and durability, saving
nearly 1/2-2/3 cost for equipments and consumables.
Two Axial Compression Column Technologies :
1.Dynamic Axial Compression:
The column and packing module are intergarted and work
together.
The piston head
pressure is monitored continuously in chromatography process, and will be
dynamically pressed tighter along the axis in
response and sensitive to minor pressure declines.
It is an optimized closed loop feedback
control system that will always maintains uniform
column resolution and performance, until the packing material is contaminated
after a long period of time.
2. Static Axial Compression:
The column and packing module(Packing Station) are seperated and work
independently.
The
column is first filled with
chromatography media packing material in the
Packing Station
with required pressure.
The column is then taken apart from the Packing Station,
and
connect to a configured chromatography system to work.
In response to pressure declines, the column piston head
could be re-pressured manually by
a planetary gear reducer or by the extension of a build-in spring.
The planetary gear reducer column has no stroke limit and material fatigue
drawbacks as that from the build-in spring type column, and does not degrade the
column resolution and performance by itself.
The Advantages of our DAC Columns :
1. The solvent
manipulation and gradient alternations all may dynamically cause the packing
particles to swell or shrink. And hence only the DAC can response rapidly to
recover from these fluctuations.
2. The DAC always dynamically maintains pressure equilibrium in axial direction
of the DAC column.
When the packing bed loosing occurs, the piston will be
pressured immediately to maintain homogenous column packing bed to maintain
stable column efficiency, and to prevent peak from tailing and shape changes.
3. The DAC
mechanism is more stable and reproducibable, and produces best product purity
and recovery rate.
4. The column packing bed can sustain stable in very long
term,
5. Routine column inner diameter from 50mm to reache a
1200mm maximum.
6. Small
packing sphere sizes as 5 microns that will almost achieve the same separation
efficiency of the analytical columns.
7. The packing
functionality have been built in the DAC system for user's own packing in the
equipment location.
8. Composed of
durable parts for prolonged applications, including gas-driven magnification
pumps, durable transmission hydraulic oil, compression piston. etc.
Application consideration for SAC Columns :
1. In case that soft packing materials
or samples may be deformed or with properties variation under larger
pressure.
2. More than one column can be packed independly with one Packing Station that
is located in user or equipment manufacturer site, and can be connected independently to HPLC Pump systems for use.
Prominent Features
and Benefits of our SAC Column:
Gelai_ABDC_SAC_Introductions.pdf
1. Global leading design and approach for
the embeded manual pressing device that performs advanced axial compression
functionality.
2. The Surface Finish of our column wall is
better than ≥
▽ 12 (ie, Ra ≤ 0.25μm) that ensuring
very good column concentricity(0.2μm), and is better than similar global
products.
We continuously and routinely cooperate with domestic and global institutions
for advanced technologies, such as the concentricity and precision polishing
technologies for column inner wall from key military units.
These efforts ensures out excellent product stability and performance.
3. Capabilities to maintain sufficiently
high internal pressure that prevent the sharp decline in column efficiency
caused by the collapsing of column bed.
In specific cases if the column bed have loosed and collapsed, the original
column pressure and column efficiency can be be restored without any tools by
just manually and gently twist on the top pressing device.
4. The column efficiency is high enough to
makes it easy to achieve linear amplification from analytical to preparation
scales. The unique column-end distribution piece design, have provided more
uniform chromatographic process for samples, with more symmetrical peak shapes.
5. With removable and both normal and
reverse directions applicable feasibilities that make user re-filling very easy.
The durable life time is much longer than other prepared column that are
implemented with fixed flange sealing mounting.
6. Eliminate the drawbacks of Spring compressed type SAC columns, such as lower
performance, large volume, and non-linear pressure decline along stroke
and spring fatigue along times.
7. The performance of SAC column is generally equal to that of DAC column in low ID ranges(<=80mmID).
8. We provide SAC column
packing services in our factories with very affordable costs.
NOTE: We can also, according to customer
requirements, manufacture customized or specialized columns that are packed with
user specified packings from various sources as Kromasil, HyperSil, Fuji, etc.
We also provide re-packing and other services for preparative columns provided
from customers .
Our Routine
SAC Specifications:
(1 inch) ID25.4mm *450mm
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(2 inch) ID50mm *450mm |
(3 inch) ID80mm *450mm
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(4 inch) ID100mm *450mm
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Customized sizes & packings |
Using same pumps to covering from SAC to DAC columns:
The
Transmission Mode of our pump systems features ± 1% Flow Accuracy or even better
to ± 0.3% in the full Flow Flow range. This prominent perpormance makes it
possible for our users to select larger pumps(for example: 500ml/min) without
minor
additional cost in the begining for thier SAC columns(it may need only <= 100ml/min
pump),
and in the future, the pumps may
switch to connect to
a 100-150mm DAC columns as well.
GL3100 Laboratory Purification system
Binary High Pressure Gradient Elution
(Option up to Quaternary Gradient--4 pumps)
Pump System:
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2 sets |
Flow range :
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0-100ml/min
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Solvent Delivering:
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Screw drive, dual piston |
Pump Material:
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316L stainless steel |
Maximum working pressure:
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6000Psi |
Flow Accuracy:
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± 1% Flow |
Repeatability:
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RRSD≤0.5% |
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Detector:
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190-740nm |
Band:
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190-740nm
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Light source:
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Deuterium lamp, Tungsten lamp
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Wavelength accuracy:
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± 1nm |
BandWidth:
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8nm
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Noise: 2 × 10-5AU
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(254nm@1mL/min@methanol) |
Drift: 15 ×10-5AU/h |
(254nm@1mL/min@methanol) |
Detection Limit: 5 × 10-9g/mL
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(254nm@1mL/min@methanol@naphthalene standard) |
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Injector:
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Manual injector with 10ml Loop |
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Column:
/td>
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25.4mm-80mm prePacked columns |
Sieve aperture:
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3-5um |
Column Material:
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316L stainless steel |
Stream Distributer:
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Multi-point forced distribution |
Column Inner Wall Finish:
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≤0.25μm(Surface Roughness) |
The system configuration can be customized: configurated
sizes can be adapted from milligram to gram-scale for research development and
production needs.
One of the new drug R&D laboratories
customer example:
Pilot scale purification system
Binary/Tertiary High/Middle Pressure Gradient Elution
Pump System:
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3 sets |
Preparation DAC Column:
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1 set
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Detector:
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1 set
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The system configuration can be customized: configurated sizes are sutable
for required production scale from gram to kg-class level.
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Solvent Delivering mode:
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Dual piston reciprocating pump |
Transmission mode:
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Screw drive
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Pump Material:
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316L stainless steel
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Specifications and pressure range:
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100ml/min pump head
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6000psi
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250ml/min pump head
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5000psi
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500 mL/min pump head
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3500 psi
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1000ml/min pump head
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2000psi
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2000ml/min pump head
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1500psi |
5000ml/min pump head
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1500psi
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10000ml/min pump head
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1500psi
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20000ml/min pump head
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1500psi |
The Transmission Mode:
Since we have integrated the international high-end
Precise Screw Transmission Mechanism that is adopted only in the high-end
instruments(the Varian(USA) also use this mode) with our own triple Close Loop
Servo Mechanism.
OOur pump systems have achieved smallest fluctuation of flow rate baseline than
other sources.
The Good system reproducibility, and low flow pulse features, is significantly
different from that of the Cam Transmission Mode from most other suppliers, and
have great advantages in terms of impulse control and precision, especially in
the high purity and gradient preparation practices.
Gelai Screw transmission |
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Varian(USA) Screw transmission
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Cam transmission |
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Dynamic Axial
Compression system(DAC)
The DAC system is composed of column cylinder, Hydraulic cylinder, piston and
pneumatic motor, is an integrated column system with packing capability. The
users could option to equip with automatic homogenizing cans, moving trays and
other configurations.
There are 150mm, 200mm, 300mm, 450mm, 600mm, DAC 800mm etc. DAC column cylinder
in diameters.
DAC_ID_50mm |
DAC_ID_150mm
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DAC_ID_500mm |
DAC Colum Miller
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Advantages of the Dynamic Axial Compression system(DAC)
1.Pressure Tolerance and Adhesiveness:
Realize the inner surface
finish of ≥▽12, namely the Surface Roughness ≤0.25μm; the concentricity of the
piston and cylindrical reach 0.2um, the piston is sealed with imported
oblique-spring seals. These features enable the column tolerance to 6000psi high
pressure without the risk of leakage, and without wearing for 50 repeatedly
filling.
2. The Uniformity and
Reproducibility of stream distribution:
Both the DAC Column inlet
and outlet ends are optimally adopted with our patented
Forced Fluid Path
Distributing technology, that enable the sample solution and mobile phrase
stream to uniformly flow
through the column, and have good peak symmetry.
3.
The packing process is easy to learn
, that uses a short time with low air compressor demand.
90 kg pressure within the cylinder can be packed using 6kg air pressure.
Detector system
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Wavelength range |
190 ~ 740nm |
Cut-off filter |
370nm |
Light sources
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Deuterium lamp, Halogen(optional) |
Wavelength accuracy |
± 1nm |
Noise |
2 × 10-5AU(254nm@1mL/min@methanol) |
Drift: |
15 ×10-5AU/h(254nm@1mL/min@methanol) |
Detection Limit
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5 ×
10-9g/mL(254nm@1mL/min@methanol@naphthalene standard) |
Examples of customers:
A FDA-approved peptide workshop
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GMP certificated peptide workshop
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A new drug R&D center & A chemical plant |
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GMP compliance system hardware |
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Material Compliance:
Pump head, pipelines, column cylinder that
direct contact with the drug, are 316L stainless steel, and the other main
support structures are 304 stainless steel, with the provision of material proof
available.
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Easy to clean,
without dead corner space: Corner
space positions in the system are arc designed, that is easy to clean and
resistant to all types of cleaning agents and disinfectants, without corrosion.
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Stable: Good
equipment performance and system reproducibility.
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Complete the URS, FAT\SAT, With customer.
Conduct DQ confirmation.
Engineers complete IQ in plant.
Complete OQ.
Complete the PQ With customer. |
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System Warranty: Two years; Deuterium lamp & Check valve: 1 year,
with normal operation after installation.
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